Enzymatic and non-enzymatic antioxidant activity of Pergularia tomentosa against carbon tetra chloride induced hepatic damage in Wistar albino rats

Vinoth Kumar, Malliga Elangovan, Raja Sekar Srinivasan


Many oxidative stress related disease are due to accumulation of free radicals in the body which causes cell injury. In this study, the enzymatic antioxidants (Superoxide dismutase, Catalase, Glutathione-s-transferase, Glutathione peroxidase, Ascorbate oxidase and Polyphenoloxidase) and non-enzymatic antioxidants (Total reduced glutathione and Vitamin C) activities were determined using Carbon tetrachloride (CCl4) rat liver as experimental model. The ethanol and chloroform extract showed noticeable increases in enzymatic antioxidant and non- enzymatic antioxidant and thus capability to scavenge the free radicals and protect against oxidative stress causing diseases. Thus, the present study indicates that the plant may be clinically valuable agent in the prevention of hepatic failure caused by CCl4 intoxication. 

Full Text:



Research guidelines for evaluating the safety and efficacy of herbal medicines, (1993) Manila, World Health organization regional office for the western pacific.

M P Kahkonen, A I Hopia, H J Vuorela, J P Rauha, K Pihlaja, T S Kujala, M Heinonen, (1999) Journal of Agricultural and Food Chemistry, 47, 3954-62.

M Valko, D Leibfritz, J Moncol, M T D Cronin, M Mazur, J Telser, (2007)International Journal of Biochemistry and Cell Biology, 39(1), 44–84.

B Halliwell, C E Cross, (1994) Environmental health perspectives, 102(Suppl 10), 5.

C A Rice-Evans, N J Miller, G Paganga, (1996) Free Radical Biology and Medicine, 20, 933–56.

K N Mahesh, M N Wickramaratne, D B M Wickramaratne, (2014) Pharmacognosy Journal, 6, 1- 3.

Z Babaamer, L Sekhari, H Al-jaber, M Al-Qudah, (2013) Annales des Sciences ET Technologie, 5 (1), 1- 4.

A Hamed, M Alberto, A Balestrieri, I Mahalel, W Springuel, C Oleszek, C Pizza, S Piacente, (2006) Journal of Natural Product, 69, 1319-22.

S Piacente, M Masullo, D Nancy, J Dewelle, A Hamed, R Kiss, T Mijatovic, (2009) Journal of Natural Product, 72, 1087 – 91.

S Heneidak, R J Grayer, G C Kite, (2006) Biochemical Systematics and Ecology, 34, 575-84.

P W C Green, N C Veitch, C Philip, S J Monique, (2011) Arthropod-Plant Interactions, 5, 219 -25.

B N Dhawan, (1995) Decade of the Brain: India/USA Research in Mental Health and Neurosciences. Rockville, 197 – 202.

OECD‐ (1996) Acute oral toxicity‐ Acute toxic class method guidelines-423, Paris.

K Das, L Samanta, G B N Chainy, (2000) Indian Journal of Biochemistry and Biophysis, 37, 201-04.

A K Sinha. (1972) Analytical Biochemistry, 47, 389-94.

T Rotruck J, A L Pope, H E Ganther, A B Swanson, D G Hafeman, W G Hoekstra, (1973) Science,; 179, 588-90.

W H Habig, M J Pabst, W B Jakoby, (1974) The Journal of Biological Chemistry, 249(22), 7130-7139.

H M Vines, M F Oberbacher, (1965)Nature, 206, 319-20.

S Sadasivam, A Manickam, (1996) Biochemical method: Polyphenol oxidase. New Age International (P) Limited, New Delhi, 2, 108-10.

A F Boyne, G L Ellman, (1972) Analytical Biochemistry, 46, 639 - 653.

G C Yen, H Y Chen, (1996) Journal of Agricultural and Food Chemistry, 47, 23-32.

D B Duncan, (1957) Biometrics, 13(2), 164-176.

J N Dhuley, S R Naik, (1997) Journal of ethnopharmacology, 56(2), 159-164.

S D Pratt, M M Kaplan, (2005)Harrison’s Principles of Internal Medicine. 16th ed. Mc Graw-Hill, 1813-15.

S Choksi, SS Patel, AK Saluja, (2000) Indian Drugs, 37 (12), 566 -569.

P Ferenci, B Dragosics, H Dittrich, H Frank, L Benda, Journal of Hepatology, 9(1), 105-113.

S Jain, VK Dixit, N Malviya, V Ambawatia, (2009) Acta Pol Pharm, 66(4), 423-428.

WF Beyer, I Fridovich, (1987) Analytical Biochemistry, 161(2), 559-66.

YL Lee, MT Yen, JL Mau, (2007) Food Chemisrty, 104(1), 1-9.

S G Sarvajeet, T Narendra, (2010) Plant Physiology and Biochemistry, 3, 1-22.

S K Jaiswal, V K Gupta, M D Ansari, N J Siddiqi, B Sharma, (2017) Toxicology Reports, 9 (4), 265-73.

Copyright (c) 2017 vinoth kumar

<Microbiology+Biotechnology+Research>Journal of Microbiology and Biotechnology Research