A comparative study of lipid profiles of cyanobacteria from two biodiversity hotspots of India (Indo-Burma and Western Ghats)

Avijeet Singh Oinam, Ojit Singh Keithellakpam, Silvia Chungkham, Indrama Thingujam, Gunapati Oinam, Romi Khangembam, Thadoi Devi Angom, Indira Devi Wangkhem, Minerva Shamjetshabam, Subhalaxmi Sharma Aribam, Onkar Nath Tiwari, Gauri Dutt Sharma

Abstract


Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotspot namely, Phormidium animale BTA 258 (P1), Leptolyngbya sp. BTA 287 (L1), Leptolyngbya sp. BTA 477 (L2), Leptolyngbya tenerrima BTA 766 (L3) and Phormidium tenue BTA 803 (P2). Fatty acids were profiled and compared with cyanobacterial fatty acids reported from Western Ghats. Cyanobacterial SFA% and MUFA% of Indo-Burma region were comparatively less as compared to Western Ghats with highest shown by heterocystous cyanobacteria (41- 98% total fatty acids). Indo Burma cyanobacterial isolates present more MUFA% in average as compared to PUFA%. PUFA% were high in P1 (11.20%) and P2 (11.36%) compared to non-heterocystous groups of both the hotspot. Butyric acid (C4:0) was significant as specific chemotaxonomic marker for the Phormidium animale and Leptolyngbya sp. of Indo-Burma region. High amount of C13:0, C14:0, C14:1, C15:0, C20:1 and C20:2 were obtained in Phormidium and Leptolyngbya of Indo-Burma region with C13:0 and C14:1 being the highest and present specifically. C15:0, C18:2n6t, C20:3n6, C22:1n9, C20:3n3, C20:4n6, C20:5n3, C22:2 and C24:1 showed diversed distribution and strain dependent at both the regions. C16:0 was found commonly in all the cyanobacteria of both the hotspots. C14:1, C15:1, C16:1, C17:1 and C18:1nt were absent in Phormidium strains of Western Ghats but present in both the Phormidium strains of Indo Burma region. Heterocystous strains of Western Ghats showed high GLA (C18:3n6) content compared to isolates of Indo-Burma region. Potential cyanobacterial candidates for chemotaxonomic marker and value added products from two hotspots of India can be ruled out from this current investigation.

 

Keywords: Comparison, Cyanobacteria, fatty acids, Indo-Burma, Western Ghats


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