Amylase production by fungi isolated from Cassava processing site

F. S. Johnson, A. K. Obeng, I. Asirifi

Abstract


Amylases are carbohydrases with greatest industrial applications. Twenty (20) fungal strains were isolated from the Aboam Cassava Processing dumpsite soil. Isolates AI020WE, AI017BN and AI009GN with largest clearing zone diameters of 59.75±0.035 mm, 57.75±1.177 mm and 56.25±0.35 mm respectively were evaluated. Submerged fermentation technique was employed for crude amylase and biomass production. There were significant differences (p<0.05) in growth rates between the three isolates. AI020WE recorded the highest growth rate of 5.38±0.21 mg/hfollowed by AI017BN and AI009GN with growth rate of 2.34±0.37 mg/h and 1.35±0.10 mg/h respectively on dry biomass weight basis.AI020WE, AI017BN and AI009GN attained their optima amylase activities of 4.49±0.398 U/ml, 2.86±2.30 U/ml and 4.51±0.20 U/ml at 60 °C, 55 °C and 65 °C respectively making them thermophilic.Isolates AI020WE and AI017BN attained optima amylase activity of 4.80±0.68 U/ml and 2.54±0.57 U/ml respectfully at pH 4 making them acidogenic. Isolates AI020WE, AI017BN and AI009GN attained their highest activities at the starch concentrations of 2%, 1.5% 4% and fermentation period of 72 hours, 42 hours and 60 hours respectively.Isolate AI020WE achieved its highest amylase activity of 11.01±0.11 U/ml at 72 hours of incubation. AI017BN and AI009GN also showed their maximum amylase activity of 3.83±0.94 U/ml and 3.24±0.11 U/ml at the 42nd and 60th hours of incubation respectively. The result shows that the cassava processing dump site soil is a repository of amylolytic fungi and some of the isolates are of industrial interest.

 

Keywords: amylase activity, clearing zone diameter, fungi, isolate


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