Emerging biofilm producing multi-drug resistant mucoid strains of Pseudomonas aeruginosa in a Rural Medical College Hospital in North Kerala

Ramakrishna Pai Jakribettu, Syed Mustaq Ahamed, Anju M M, Safeera M I V, Ashthami V Chandran


Pseudomonas aeruginosahas emerged as the multi-drug pathogen in nosocomial infections causing Urinary tract infections, pneumonia, surgical site infections, burns infections. The emergence of drug resistance is due to production of biofilm and thus significantly reducing the penetration of antibiotic through the glycocalyx matrix. In this study, we have studied the antibiogram and biofilm production in both mucoid and non-mucoid strains of Pseudomonas aeruginosaisolated from nosocomial infections. Sample from patients suspected with Nosocomial infections were collected and processed according to standard procedure and antibiotic susceptibility was done according to CLSI guidelines and subjected for biofilm production by tissue culture plate and test tube method. A total of 64Pseudomonasaeruginosa were isolated from different clinical samples. Among 64, 36 (56.25%) was isolated from sputum samples and least 2 (3.12%) was from blood samples. Among the 64 isolates of Pseudomonas aeruginosa, 53 (82.81%) were non-mucoid and 9 (17.19%) were mucoid strains. The Mucoidstrains were more resistant to fluoroquinolones (45%) , followed by β lactum and monobactum group ( 27%), and aminoglycosides (18%). Among the isolates tested strong biofilm producers were 41(64%) and moderate biofilm producers were 23(36%) by Tissue culture plate method. The Mucoid strains of Pseudomonas aeruginosa were multidrug resistant, which is a concern in the nosocomial infections. These can cause increase in morbidity and mortality due to nosocomial infection.


Key words: Mucoid Pseudomonas, Nosocomial Infections, Biofilm and resistance

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