Antibacterial activities of different solvent extracts of Neuboldia Levis parts on some gram positive and negative uropathogenic bacterial isolates

Orhue P. O., Momoh ARM, Igumbor E. O, Esumeh F. I.

Abstract


Considering the assertion that medicinal plants constitute a continuous source of new compounds with potential to act against multi-resistant bacteria, this present investigates the antimicrobial activity of various extracts of the leaves and stem bark of Newbouldia laevis on human urinary tract bacteria isolates. Following standard laboratory procedures, the leaves and back extracts of Newbouldia laevis were studied on Gram positive bacteria (Staphylococcus aureus) and two strains of Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The degree of antibiotic potentials varies with the extraction solvents (water, 1%HCL, ethanolic, acetone and petroleum ether) but not with the parts of the plant. S. aureus and E.coli were more sensitive to Neuboldia levis leaves and bark than P. aeruginosa. Compared to the five extraction solvents, leaf and bark extracts of Neuboldia levis in acetone and petroleum ether showed the best antimicrobial potentials. Neuboldia levis leaf and back extracts demonstrated bactericidal potential against both gram-positive and gram-negative bacteria and could be useful in the inhibition of human urinary tract biotic bacterium.

 

Key words: Herbs, Neuboldia levis, Antimicrobial, Extraction solvents.


Full Text:

PDF

References


Karaman L, Sahin F, Gulluce M, Ogutcu H, Sngul M, Adiguzel A. (2003). J Ethnopharmacol; 85: 231-235.

Nino J, Navaez DM, Mosquera OM, Correa YM. (2006). Brazilian J MIcrobiol 37: 566-570.

Schinor EC, Salvador MJ, Ito IY, Dias DA. (2007). Brazilian J MIcrobiol; 38: 145-149.

Essawi T, Srour M. (2000). J Ethanopharmacol 46: 343-349.

Orhue P.O., Edomwande E.C., Igbinosa E., Momoh A.R.M. and Asekomhe O. (2014). Inter. J. Herbs Pharmaco.

Res., 3(1): 24 – 29

Racio MC, Rios JC, Villar A. (1989). Phytotherapy Res 3(4): 117-125.

Arbonnier, M. (2004) Trees, Shrubs and Lianas of West African Dry zones, CIRAD, Margraf Publishers GMBH MNHN, Cote d’ivories P.194

Akunyili, D.N. (2000) Anticonvulsant Activity of the Ethanolic extract of Newbouldia laevis 2nd NAAP Scientific Conference. P.155-158.

Iwu, M.M (2000). Handbook of African Medicinal Plants. CRC Press, Inc. London. P.19.

Gorman R, Kaloga M, Ferreira D, Bergenthai D, Kolodziej H. (2003). Phytochem.; 64:583-587.

Eyong OK, Krohn K, Hussain H, Folefoc NG, Nkengfack AE, Schulz B, Hu Q (2005). Chem Pharm Bull; 53: 616–619.

Gill LS. (1992). Ethnomedical uses of plants in Nigeria. University of Benin Press, Benin City; pp 174-175.

Al-Bayati FA, Al-Mola HF. (2008). J Zhejiang Univ. Sci. B; 9(2): 154–159.

Orhue .P.O. (2004): PhD THESIS Ambrose Alli University, Nigeria.

Usman H, Osuji JC. (2007). Afr J Trad Compl & Alt Med.; 4(4): 476-480.

Usman H, Haruna AK, Akpulu IN, Ilyas M, Ahmadu AA, Musa YM. (2005). J. Trop. Biosci.; 5 (2): 72-76.

Chukwujeku JC, Staden JV, Smith P. SA. (2005). J Bot.; 71 (3&4): 316–325.

Gomathi, S.,Ambikapathy,V.and Panneerselvam, A. (2011). J. Microbiol. Biotech. Res.; 1 (2):8-13

Adesanya SA, Nia R, Fontaine C, Pais M (1994). Phytochemistry; 35: 1053–1055.

Houghton PJ, Pandey R, Hawkes JE, (1994) Phytochemistry; 35: 1602–1603.

Gafner S, Wolfender JL, Nianga M, Hostettmann K (1997). Phytochemistry; 44: 687–690.

Aladesanmi AJ, Nia R, Nahrstedt A (1998). Planta Med; 64: 90–91.

Okeke AS, Okeke MI, Iroegbu CU, Ebo PU (2002) Phytother Res; 16: 174– 179.

Eyong OK, Folefoc NG, Kuete V, Beng, VP, Hussain H, Krohn K, Nkengfack AE, Ramadan SSS, Hoerauf A (2006). Phytochemistry; 67: 605–609.

Germann K, Kaloga M, Ferreira D, Marais JP, Kolodziej H. (2006). Phytochem; 67(8): 805–811.

Cowan MM (1999). Clin Microbiol Rev; 12: 564–582.

Rojas A, Hernandez L, Perreda-Mirranda R, Matg R (1992). J Ethnopharmacol; 35: 275–283.

Perrett S, Whitfield PJ, Sanderson L, Bartlrtt A (1995). J Ethnopharmacol; 47: 49–54.

Stern JL, Hagerman AE, Steinberg PD, Mason PK (1996). J Chem Ecol; 22: 1887–1899.




Copyright (c) 2017 Journal of Microbiology and Biotechnology Research




<Microbiology+Biotechnology+Research>Journal of Microbiology and Biotechnology Research