A study on Biosynthesis of Iron nanoparticles by Pleurotus sp.

Harajyoti Mazumdar, Nabanita Haloi

Abstract


Productions of nanoparticles by using microbes are a new area of interest. Pleurotus sp was allowed for biosynthesis of iron nanoparticles in a culture media. The fungus was allowed to grow in 2x10-4 M FeSO4 solutions for 72 hours. U.V-Vis spectra analysis helps us to know about formations of nanoparticles at 226 nm and 276 nm wavelength. Involvement of proteins in culture was determined by spectral analysis of broth culture at different interval of times at 265 nm. FTIR analysis detects the presence of functional groups in binding of particles with biomass. There was a shift in case of treated cells indicating participations of proteins in more quantity. TEM images reflect depositions of particles in both inside as well as outside indicating the biosynthesis process. However such depositions are absent in case of control sample. XRF of control samples donot shows any iron elements but was present in treated mycelium. Some others elements were also detected, it may be due to present in culture media. The transport of iron particles may be due to presence of Siderophores. Iron transport molecules like hydroxamates are mainly present in fungi. They bind the complex molecules and transport them inside of cells.

 

Keywords: Nanoparticles, Biosynthesis, Fourier Transform Infra Red spectrophotometer (FTIR), Transmission Electron Microscope (TEM), X-Ray Fluorescence (XRF).

 

 


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