In-vitro evaluation of herbal and chemical agents in the management of dandruff

  • Naveen S Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
  • Karthika S Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
  • Sentila R Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
  • Mahenthiran R Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
  • Michael A Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.

Abstract

Dandruff, a universal non-inflammatory scaling condition restricted to the scalp is caused by the lipophilic yeasts of the genus, Malassezia. This study investigated in in-vitro, the anti-microbial claims of some of the current chemical and herbal products in the market. A standard isolate of Malassezia furfur was used for the in-vitro study and maintained in Sabouraud’s dextrose agar supplemented with corn oil. Multiple agents utilised in over-the-counter products claiming anti-dandruff activity were selected based on literature for this study. Among the shampoos tested, Zinc Pyrithione based shampoo’s showed a zone of inhibition of 37mm and shampoo’s containing tea tree oil showed a zone of inhibition of 39mm. The Minimum inhibitory concentration of the shampoo actives against Malassezia furfur were studied in-vitro by agar dilution method, where 10mg/ml of Zinc Pyrithione showed effective inhibition followed by 100mg/ml for tea tree oil. Zinc Pyrithione and Ketaconazole based shampoos recorded good antidandruff activity among synthetic ingredients with an ability to reduce the growth of the test organism by 67% and 44% respectively. Teatree oil scored good activity among herbal ingredients with the recorded 78% reduction in microbial growth. Synthetic drugs have the shortcomings of poor efficacy and recurrence, so herbal agents can be an effective alternative. Keywords: Dandruff, Malassezia furfur, Chemical agents, Herbal agents.

Author Biographies

Naveen S, Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Karthika S, Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Sentila R, Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Mahenthiran R, Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Michael A, Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.
Dept of Microbiology, PSG College of Arts & Science, Coimbatore, India.

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Published
2017-03-31
Section
Article