Antibiogram and plasmid profiling of E.coli isolated from cattle

Lullu P. K, Lali Growther


Escherichia coli are a member of the genus Escherichia within the family Enterobacteriaceae and are comprised of mostly motile, gram negative bacilli. In this study, 40 faecal samples were collected from cattle, which were confirmed by the biochemical test. The antimicrobial susceptibility was tested for all the 40 E.coli isolates towards 18 antibiotics. The resistance was observed commonly to fostomycin, piperacillin, nalidixic acid, amoxyclav, ampicillin, aztreonam, and ceftazidime. Plasmid profiling of the samples were also performed.


Key words: E.coli, Antibiotic resistance, plasmids, multidrug resistance.

Full Text:



Edwards P.R, W.H.Ewing. 1972. Identification of Enterobacteriaciae Minneapolis, Minnesota: Burgess Publishing Company.

Lior H. 1996. Classification of E.coli. In Escherichia coli in Domestic animals and Humans.Edited by Gyles CL.Wallingford,United Kingdom:CAB International;31-72.

Vial P.A.1988. J Infect Dis.158:70-79.

Hung Vu-Khac, Nancy A.Cornick.2008. Veterinary Microbiology 126:356-363.

Bergey's Manual of Systematic Bacteriology. (2nd ed.). 2005. New York: Springer. Berkhoff, H.A., Vinal, A.C., Congo red medium to distinguish between invasive and noninvasive Escherichia coli pathogenic for poultry. Avian Dis., 1986, 30: 117-121.

Clinical and Laboratory Standards Institute. Analysis and presentation of cumulative antimicrobial susceptibility test data; approved guideline. 2nd ed. CLSI document M39-A2. Wayne (PA): CLSI; 2005.

Sambrook,J.,E.F.Fritsch and T Maniatis.1989.Molecular cloning: a laboratory manual ,2nd ed.,Cold Spring Harbour Laboratory Press,Cold Spring Harbour.1:6.9-6.19.

Asis Khan, S.C.Das, T.Ramamurthy, A.Sikhdar, J.Khanam, S.Yamasaki, Y.Takeda, G.Balakrish Nair.2002. J.Clin.Microbiol 40:2009-2015.

Markku Keskimaki, Marjut Saari, Tarja Heiskanen and Anja Siitonen.1998. J.Clin.Microbiol, 36:3641-3646.

Shaohua Z, David.G.White, Beilei Ge, Sherry Ayers, Sharon Friedman, Linda English, David Wagner, Stuart Gaines and Jianghong Meng.2001. Appl.Environ.Microbiol.67:1558-156.

Marjut Eklund, Flemming Scheutz, and Anja Siitonen.2001. J.Clin.Microbiol 39:2829-2834.

Copyright (c) 2017 Journal of Microbiology and Biotechnology Research

<Microbiology+Biotechnology+Research>Journal of Microbiology and Biotechnology Research